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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPN1 Polyclonal antibody (13385-1-AP)

    WB analysis using 13385-1-AP (same clone as 13385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CPN1 Polyclonal antibody (13385-1-AP)

    WB analysis of Raji using 13385-1-AP (same clone as 13385-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPN1 Polyclonal antibody (13385-1-AP)

    IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPN1 Polyclonal antibody (13385-1-AP)

    IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using CPN1 Polyclonal antibody (13385-1-AP)

    IF Staining of COLO 320 using 13385-1-AP (same clone as 13385-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CPN1 antibody (13385-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CPN1 Polyclonal antibody (13385-1-AP)

    IF Staining of HepG2 using 13385-1-AP (same clone as 13385-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CPN1 antibody (13385-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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CPN1 Polyclonal antibody, PBS Only

CPN1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13385-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13385-1-PBS targets CPN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPN1 fusion protein Ag4035 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase N, polypeptide 1
Calculated molecular weight 458 aa, 52 kDa
Observed molecular weight 40~50 kDa
GenBank accession numberBC027897
Gene Symbol CPN1
Gene ID (NCBI) 1369
RRIDAB_2292159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15169
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.