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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPN1 Polyclonal antibody (31940-1-AP)

    WB analysis using 31940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31940-1-AP (CPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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CPN1 Polyclonal antibody

CPN1 Polyclonal Antibody for WB, ELISA
Cat No. 31940-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Kininase-1, Kininase 1, EC:3.4.17.3, CPN, Arginine carboxypeptidase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31940-1-AP targets CPN1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPN1 fusion protein Ag34806 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase N, polypeptide 1
Calculated molecular weight 458 aa, 52 kDa
Observed molecular weight 40~50 kDa
GenBank accession numberBC027897
Gene Symbol CPN1
Gene ID (NCBI) 1369
RRIDAB_3670152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP15169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.

Protocols

Product Specific Protocols
WB protocol for CPN1 antibody 31940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols