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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPN2 Polyclonal antibody

CPN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27160-1-AP

Synonyms

ACBP, Carboxypeptidase N subunit 2, CPN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPN2 Polyclonal antibody (27160-1-AP)

    WB analysis using 27160-1-AP

    human plasma tissue were subjected to SDS PAGE followed by western blot with 27160-1-AP (CPN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using CPN2 Polyclonal antibody (27160-1-AP)

    IHC staining of human liver using 27160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27160-1-AP (CPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CPN2 Polyclonal antibody (27160-1-AP)

    IHC staining of human liver using 27160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27160-1-AP (CPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27160-1-AP targets CPN2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPN2 fusion protein Ag25801 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase N, polypeptide 2
Calculated molecular weight 545 aa, 61 kDa
Observed molecular weight83-90 kDa
GenBank accession numberBC031569
Gene symbol CPN2
Gene ID (NCBI) 1370
RRIDAB_3085929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain (PMID:17157876). CPN2 also performs a crucial biological function in the invasion and migration of breast cancer and can be used as a biomarker for effective diagnosis and treatment of breast cancer.

Protocols

Product Specific Protocols
WB protocol for CPN2 antibody 27160-1-APDownload protocol
IHC protocol for CPN2 antibody 27160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols