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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPN2 Polyclonal antibody, PBS Only

CPN2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27160-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPN2 Polyclonal antibody (27160-1-AP)

    WB analysis using 27160-1-AP (same clone as 27160-1-PBS)

    human plasma tissue were subjected to SDS PAGE followed by western blot with 27160-1-AP (CPN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27160-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CPN2 Polyclonal antibody (27160-1-AP)

    IHC staining of human liver using 27160-1-AP (same clone as 27160-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27160-1-AP (CPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27160-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CPN2 Polyclonal antibody (27160-1-AP)

    IHC staining of human liver using 27160-1-AP (same clone as 27160-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27160-1-AP (CPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27160-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27160-1-PBS targets CPN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPN2 fusion protein Ag25801 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase N, polypeptide 2
Calculated molecular weight 545 aa, 61 kDa
Observed molecular weight83-90 kDa
GenBank accession numberBC031569
Gene symbol CPN2
Gene ID (NCBI) 1370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain (PMID:17157876). CPN2 also performs a crucial biological function in the invasion and migration of breast cancer and can be used as a biomarker for effective diagnosis and treatment of breast cancer.