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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPOX Polyclonal antibody, PBS Only

CPOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16724-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis using 16724-1-AP (same clone as 16724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of K-562 using 16724-1-AP (same clone as 16724-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of human liver using 16724-1-AP (same clone as 16724-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of K-562 using 16724-1-AP (same clone as 16724-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of mouse liver using 16724-1-AP (same clone as 16724-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of rat liver using 16724-1-AP (same clone as 16724-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CPOX Polyclonal antibody (16724-1-AP)

    IP experiment of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

    IP result of anti-CPOX (IP:16724-1-AP, 4ug; Detection:16724-1-AP 1:1000) with HepG2 cells lysate 1800ug. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    IHC staining of human liver using 16724-1-AP (same clone as 16724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    IHC staining of human liver using 16724-1-AP (same clone as 16724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CPOX Polyclonal antibody (16724-1-AP)

    IHC staining of human testis using 16724-1-AP (same clone as 16724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CPOX Polyclonal antibody (16724-1-AP)

    IHC staining of human testis using 16724-1-AP (same clone as 16724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16724-1-PBS targets CPOX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPOX fusion protein Ag10164 相同性解析による交差性が予測される生物種
Full Name coproporphyrinogen oxidase
Calculated molecular weight 454 aa, 50 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC017210
Gene symbol CPOX
Gene ID (NCBI) 1371
RRIDAB_2084231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.