CPSF7 Polyclonal antibody

Name: CPSF7 Polyclonal antibody
Brand: Proteintech
SKU: 55195-1-AP

CPSF7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55195-1-AP

Synonyms

CPSF 59 kDa subunit, CPSF7, FLJ12529

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 55195-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 55195-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55195-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55195-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55195-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 55195-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 55195-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 55195-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 55195-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 55195-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 55195-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 55195-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (55195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 55195-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse brain tissue
Positive IHC detected in	human gliomas tissue, human skin tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

55195-1-AP targets CPSF7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	pre-mRNA cleavage factor I, 59 kDa subunit
Calculated molecular weight	56 kDa
Observed molecular weight	52 kDa, 56 kDa
GenBank accession number	NM_001136040
Gene symbol	CPSF7
Gene ID (NCBI)	79869
RRID	AB_10858797
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.

Protocols

Product Specific Protocols
WB protocol for CPSF7 antibody 55195-1-AP	Download protocol
IHC protocol for CPSF7 antibody 55195-1-AP	Download protocol
IF protocol for CPSF7 antibody 55195-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Cell Rep CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation Authors - Susu Liu View Article
human	CoIP	J Mol Cell Biol U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors Authors - Zhijie Hu View Article
		PLoS Pathog Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses Authors - Yong Ge View Article
human	WB	Mol Cell 2'-O-methylation at internal sites on mRNA promotes mRNA stability Authors - Yanqiang Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPSF7 Polyclonal antibody

CPSF7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 55195-1-AP

WB analysis of HEK-293 using 55195-1-AP

WB analysis of mouse brain using 55195-1-AP

WB analysis of mouse brain using 55195-1-AP

WB analysis of HeLa using 55195-1-AP

IHC staining of human gliomas using 55195-1-AP

IHC staining of human gliomas using 55195-1-AP

IHC staining of human testis using 55195-1-AP

IHC staining of human testis using 55195-1-AP

IHC staining of human skin using 55195-1-AP

IHC staining of human skin using 55195-1-AP

IF Staining of A431 using 55195-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation

U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors

Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses

2'-O-methylation at internal sites on mRNA promotes mRNA stability