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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis using 55195-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis of HEK-293 using 55195-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis of mouse brain using 55195-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis of mouse brain using 55195-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis of HeLa using 55195-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human gliomas using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human gliomas using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human testis using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human testis using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human skin using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human skin using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CPSF7 Polyclonal antibody (55195-1-AP)

    IF Staining of A431 using 55195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (55195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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CPSF7 Polyclonal antibody

CPSF7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55195-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

CPSF 59 kDa subunit, CPSF7, FLJ12529

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55195-1-AP targets CPSF7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name pre-mRNA cleavage factor I, 59 kDa subunit
Calculated molecular weight 56 kDa
Observed molecular weight 52 kDa, 56 kDa
GenBank accession numberNM_001136040
Gene Symbol CPSF7
Gene ID (NCBI) 79869
RRIDAB_10858797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.

Protocols

Product Specific Protocols
WB protocol for CPSF7 antibody 55195-1-APDownload protocol
IHC protocol for CPSF7 antibody 55195-1-APDownload protocol
IF protocol for CPSF7 antibody 55195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Rep

CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation

Authors - Susu Liu
View Article
humanCoIP

J Mol Cell Biol

U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors

Authors - Zhijie Hu
View Article

PLoS Pathog

Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses

Authors - Yong Ge
View Article
humanWB

Mol Cell

2'-O-methylation at internal sites on mRNA promotes mRNA stability

Authors - Yanqiang Li
View Article