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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPT1A Monoclonal antibody (66039-3-Ig)

    WB analysis using 66039-3-Ig (same clone as 66039-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CPT1A Monoclonal antibody (66039-3-Ig)

    WB analysis of mouse liver using 66039-3-Ig (same clone as 66039-3-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CPT1A Monoclonal antibody (66039-3-Ig)

    WB analysis using 66039-3-Ig (same clone as 66039-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CPT1A Monoclonal antibody (66039-3-Ig)

    WB analysis using 66039-3-Ig (same clone as 66039-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CPT1A Monoclonal antibody (66039-3-Ig)

    IHC staining of mouse heart using 66039-3-Ig (same clone as 66039-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CPT1A Monoclonal antibody (66039-3-Ig)

    IHC staining of mouse heart using 66039-3-Ig (same clone as 66039-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using CPT1A Monoclonal antibody (66039-3-Ig)

    IHC staining of rat heart using 66039-3-Ig (same clone as 66039-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using CPT1A Monoclonal antibody (66039-3-Ig)

    IHC staining of rat heart using 66039-3-Ig (same clone as 66039-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CPT1A Monoclonal antibody (66039-3-Ig)

    IHC staining of mouse skeletal muscle using 66039-3-Ig (same clone as 66039-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CPT1A Monoclonal antibody (66039-3-Ig)

    IHC staining of mouse skeletal muscle using 66039-3-Ig (same clone as 66039-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

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CPT1A Monoclonal antibody, PBS Only

CPT1A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66039-3-PBS
Clone No.5D2B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform, Carnitine O-palmitoyltransferase 1, liver isoform

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66039-3-PBS targets CPT1A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CPT1A fusion protein Ag7745 相同性解析による交差性が予測される生物種
Full Name carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight 88 kDa
Observed molecular weight86 kDa
GenBank accession numberBC000185
Gene Symbol CPT1A
Gene ID (NCBI) 1374
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50416
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.