Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 22170-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 22170-1-AP
Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 22170-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 22170-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 22170-1-AP
IP result of anti-CPT1B-specific (IP:22170-1-AP, 4ug; Detection:22170-1-AP 1:600) with mouse heart tissue lysate 3600 ug.
IP experiment of mouse skeletal muscle using 22170-1-AP
IP result of anti-CPT1B-specific (IP:22170-1-AP, 4ug; Detection:22170-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.
IHC staining of mouse heart using 22170-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 22170-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 22170-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 22170-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CPT1B-specific antibody (22170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 22170-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CPT1B-specific antibody (22170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

CPT1B-specific Polyclonal antibody

CPT1B-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 22170-1-AP

Publications(67)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

CPT1B, CPT1 M, Carnitine palmitoyltransferase I-like protein, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine O-palmitoyltransferase 1, muscle isoform

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (67)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected in	mouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected in	mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 63 publications below
IHC	See 3 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

22170-1-AP targets CPT1B-specific in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, zebrafish, sheep, ewes
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CPT1B-specific fusion protein Ag17840 相同性解析による交差性が予測される生物種
Full Name	carnitine palmitoyltransferase 1B (muscle)
Calculated molecular weight	567 aa, 64 kDa
Observed molecular weight	75-85 kDa
GenBank accession number	BC131570
Gene Symbol	CPT1B
Gene ID (NCBI)	1375
RRID	AB_2713959
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92523
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.

Protocols

Product Specific Protocols
WB protocol for CPT1B-specific antibody 22170-1-AP	Download protocol
IHC protocol for CPT1B-specific antibody 22170-1-AP	Download protocol
IF protocol for CPT1B-specific antibody 22170-1-AP	Download protocol
IP protocol for CPT1B-specific antibody 22170-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Cell Metab A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis. Authors - Hui Peng View Article
mouse	WB	Cell Metab Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis. Authors - Judith Simcox KD Validated View Article
human	WB	Adv Sci (Weinh) m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia Authors - Zijuan Wu View Article
mouse	WB	Nat Commun Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism. Authors - Mahtab Tavasoli View Article
human	WB	J Biomed Sci Adipocyte pyroptosis occurs in omental tumor microenvironment and is associated with chemoresistance of ovarian cancer Authors - Chang-Ni Lin View Article
human	WB	Biomaterials LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells. Authors - Myeong-Hwa Song View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22170-1-AP

WB analysis of rat skeletal muscle using 22170-1-AP

WB analysis of mouse heart using 22170-1-AP

WB analysis of mouse skeletal muscle using 22170-1-AP

IP experiment of mouse heart using 22170-1-AP

IP experiment of mouse skeletal muscle using 22170-1-AP

IHC staining of mouse heart using 22170-1-AP

IHC staining of mouse heart using 22170-1-AP

IHC staining of mouse heart using 22170-1-AP

IF Staining of mouse heart using 22170-1-AP

IF Staining of mouse heart using 22170-1-AP

CPT1B-specific Polyclonal antibody

CPT1B-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis.

m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia

Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism.

Adipocyte pyroptosis occurs in omental tumor microenvironment and is associated with chemoresistance of ovarian cancer

LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.