CPT1C-specific Polyclonal antibody
CPT1C-specific Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12969-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human gliomas tissue, human testis tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IF | See 1 publications below |
Product Information
12969-1-AP targets CPT1C-specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CPT1C-specific fusion protein Ag3649 相同性解析による交差性が予測される生物種 |
| Full Name | carnitine palmitoyltransferase 1C |
| Calculated molecular weight | 792 aa, 90 kDa |
| Observed molecular weight | 70-82 kDa |
| GenBank accession number | BC029104 |
| Gene symbol | CPT1C |
| Gene ID (NCBI) | 126129 |
| RRID | AB_2084844 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CPT1C belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1C is an active forms of related brain-type carnitine palmitoyltransferase I. CPT1C may be a regulated target of malonyl-CoA that relays the "malonyl-CoA signal" in hypothalamic neurons that express the orexigenic and anorexigenic neuropeptides that regulate food intake and peripheral energy expenditure (PMID: 17018521). This antibody specifically recognizes CPT1C, and does not cross-react with CPT1A or CPT1B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CPT1C-specific antibody 12969-1-AP | Download protocol |
| IHC protocol for CPT1C-specific antibody 12969-1-AP | Download protocol |
| IP protocol for CPT1C-specific antibody 12969-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Etomoxir Actions on Regulatory and Memory T Cells Are Independent of Cpt1a-Mediated Fatty Acid Oxidation. | ||
Cell Rep Med Multiomic analysis identifies CPT1A as a potential therapeutic target in platinum-refractory, high-grade serous ovarian cancer. | ||
Proc Natl Acad Sci U S A Important roles of brain-specific carnitine palmitoyltransferase and ceramide metabolism in leptin hypothalamic control of feeding. | ||
JAMA Neurol Mutation in CPT1C Associated With Pure Autosomal Dominant Spastic Paraplegia. | ||
Cell Rep HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression. | ||
FASEB J Ghrelin effects on neuropeptides in the rat hypothalamus depend on fatty acid metabolism actions on BSX but not on gender. |
