CD21 Monoclonal antibody
CD21 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1D9A3
Cat no : 66701-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66701-1-Ig targets CD21 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD21 fusion protein Ag18058 相同性解析による交差性が予測される生物種 |
| Full Name | complement component (3d/Epstein Barr virus) receptor 2 |
| Calculated molecular weight | 1092 aa, 119 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC136394 |
| Gene symbol | CD21 |
| Gene ID (NCBI) | 1380 |
| RRID | AB_2882054 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD21 antibody 66701-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
