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CRABP2 Monoclonal antibody

CRABP2 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A5F3

Cat no : 66468-1-Ig

Synonyms

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

Validation Data Gallery

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  • Western Blot (WB) analysis of T-47D cells using CRABP2 Monoclonal antibody (66468-1-Ig)

    WB analysis of T-47D using 66468-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CRABP2 Monoclonal antibody (66468-1-Ig)

    WB analysis of HEK-293 using 66468-1-Ig

    WB result of CRABP2 antibody (66468-1-Ig; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of MCF-7 cells using CRABP2 Monoclonal antibody (66468-1-Ig)

    WB analysis of MCF-7 using 66468-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skin tissue using CRABP2 Monoclonal antibody (66468-1-Ig)

    WB analysis of rat skin using 66468-1-Ig

    rat skin tissue were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig skin tissue using CRABP2 Monoclonal antibody (66468-1-Ig)

    WB analysis of pig skin using 66468-1-Ig

    pig skin tissue were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CRABP2 Monoclonal antibody (66468-1-Ig)

    IHC staining of human ovary tumor using 66468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CRABP2 Monoclonal antibody (66468-1-Ig)

    IHC staining of human ovary tumor using 66468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human skin cancer tissue using CRABP2 Monoclonal antibody (66468-1-Ig)

    IF Staining of human skin cancer using 66468-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using CRABP2 Monoclonal antibody (66468-1-Ig)

    FC experiment of MCF-7 using 66468-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CRABP2 (66468-1-Ig, Clone:1A5F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inT-47D cells, HEK-293 cells, MCF-7 cells, pig skin tissue, rat skin tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman skin cancer tissue
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66468-1-Ig targets CRABP2 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRABP2 fusion protein Ag0309 相同性解析による交差性が予測される生物種
Full Name cellular retinoic acid binding protein 2
Calculated molecular weight 16 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC001109
Gene symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2881836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Protocols

Product Specific Protocols
WB protocol for CRABP2 antibody 66468-1-IgDownload protocol
IHC protocol for CRABP2 antibody 66468-1-IgDownload protocol
IF protocol for CRABP2 antibody 66468-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Cell Death Dis

Upregulation of CRABP2 by TET1-mediated DNA hydroxymethylation attenuates mitochondrial apoptosis and promotes oxaliplatin resistance in gastric cancer

Authors - Xiaolong Tang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Linyi (Verified Customer) (01-17-2019)

We use this antibody on porcine chondrocytes lysate, 1/1000 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: chondrocytes, pig
CRABP2 Antibody Western Blot, validation (1/1000 dilution) in chondrocytes, pig (Cat no:66468-1-Ig)