Featured Product
KD/KO Validated

RAIDD Polyclonal antibody

Name: RAIDD Polyclonal antibody
Brand: Proteintech
SKU: 10401-1-AP

RAIDD Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10401-1-AP

Synonyms

CRADD, RAIDD

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of K-562 using 10401-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10401-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10401-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10401-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10401-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10401-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10401-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10401-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10401-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10401-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 10401-1-AP
IP result of anti-RAIDD (IP:10401-1-AP, 3ug; Detection:10401-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.
IHC staining of human colon cancer using 10401-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10401-1-AP (RAIDD antibody) at dilution of 1:100 (under 25x lens).

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Cat No. 10401-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, HEK-293 cells, HeLa cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, mouse testis tissue, Raji cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below

Product Information

10401-1-AP targets RAIDD in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAIDD fusion protein Ag0630 相同性解析による交差性が予測される生物種
Full Name	CASP2 and RIPK1 domain containing adaptor with death domain
Calculated molecular weight	23 kDa
Observed molecular weight	23 kDa
GenBank accession number	BC017042
Gene symbol	CRADD
Gene ID (NCBI)	8738
RRID	AB_2085477
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAIDD, also named as CRADD, is an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. CRADD interacts with BCL10 through its caspase recruitment domain and suppresses interactions between BCL10 and CARMA1. It is a negative regulator of the CARMA1 signalosome and suppressor of Th1-and Th17-mediated inflammatory responses.

Protocols

Product Specific Protocols
WB protocol for RAIDD antibody 10401-1-AP	Download protocol
IHC protocol for RAIDD antibody 10401-1-AP	Download protocol
IP protocol for RAIDD antibody 10401-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Dev Cell E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration. Authors - Valentina C Sladky KD Validated View Article
mouse	WB	EMBO Rep PIDDosome-induced p53-dependent ploidy restriction facilitates hepatocarcinogenesis. Authors - Valentina C Sladky View Article
mouse	WB	J Immunol Cutting edge: the "death" adaptor CRADD/RAIDD targets BCL10 and suppresses agonist-induced cytokine expression in T lymphocytes. Authors - Lin Qing Q KO Validated View Article
human, mouse	WB	J Biol Chem The Adaptor CRADD/RAIDD Controls Activation of Endothelial Cells by Proinflammatory Stimuli. Authors - Huan Qiao View Article
mouse	WB	Cell Metab PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis Authors - Ju Youn Kim KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAIDD Polyclonal antibody

RAIDD Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of K-562 using 10401-1-AP

WB analysis of mouse liver using 10401-1-AP

WB analysis of HEK-293 using 10401-1-AP

WB analysis of HeLa using 10401-1-AP

WB analysis of Raji using 10401-1-AP

WB analysis of mouse heart using 10401-1-AP

WB analysis of mouse skeletal muscle using 10401-1-AP

WB analysis of mouse testis using 10401-1-AP

WB analysis of MCF-7 using 10401-1-AP

WB analysis of mouse kidney using 10401-1-AP

IP experiment of mouse kidney using 10401-1-AP

IHC staining of human colon cancer using 10401-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration.

PIDDosome-induced p53-dependent ploidy restriction facilitates hepatocarcinogenesis.

Cutting edge: the "death" adaptor CRADD/RAIDD targets BCL10 and suppresses agonist-induced cytokine expression in T lymphocytes.

The Adaptor CRADD/RAIDD Controls Activation of Endothelial Cells by Proinflammatory Stimuli.

PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis