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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse eye tissue using CRB1 Polyclonal antibody (26598-1-AP)

    IHC staining of mouse eye using 26598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CRB1 Polyclonal antibody (26598-1-AP)

    IHC staining of mouse eye using 26598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRB1 Polyclonal antibody (26598-1-AP)

    IHC staining of mouse brain using 26598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRB1 Polyclonal antibody (26598-1-AP)

    IHC staining of mouse brain using 26598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CRB1 Polyclonal antibody (26598-1-AP)

    IHC staining of mouse eye using 26598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CRB1 Polyclonal antibody (26598-1-AP)

    IHC staining of mouse eye using 26598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CRB1 Polyclonal antibody (26598-1-AP)

    IF Staining of SH-SY5Y using 26598-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRB1 antibody (26598-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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CRB1 Polyclonal antibody

CRB1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 26598-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

IHC, IF/ICC, ELISA

Synonyms

CRB1, Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, RP12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse eye tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26598-1-AP targets CRB1 in IHC, IF/ICC, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRB1 fusion protein Ag24512 相同性解析による交差性が予測される生物種
Full Name crumbs homolog 1 (Drosophila)
Calculated molecular weight 1406 aa, 154 kDa
Observed molecular weight150 kDa
GenBank accession numberBC136271
Gene Symbol CRB1
Gene ID (NCBI) 23418
RRIDAB_2918104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).

Protocols

Product Specific Protocols
IHC protocol for CRB1 antibody 26598-1-APDownload protocol
IF protocol for CRB1 antibody 26598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols