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- KD/KO Validated
CRBN Polyclonal antibody
CRBN Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11435-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, COLO 320 cells, human brain tissue, human heart tissue, A375 cells, mouse liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | COLO 320 cells, A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 19 publications below |
| IHC | See 4 publications below |
| IP | See 2 publications below |
Product Information
11435-1-AP targets CRBN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CRBN fusion protein Ag1951 相同性解析による交差性が予測される生物種 |
| Full Name | cereblon |
| Calculated molecular weight | 442 aa, 51 kDa |
| Observed molecular weight | 51-55 kDa |
| GenBank accession number | BC017419 |
| Gene symbol | CRBN |
| Gene ID (NCBI) | 51185 |
| RRID | AB_2085739 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRBN antibody 11435-1-AP | Download protocol |
| IHC protocol for CRBN antibody 11435-1-AP | Download protocol |
| IF protocol for CRBN antibody 11435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science DRUG DEVELOPMENT. Phthalimide conjugation as a strategy for in vivo target protein degradation.
| ||
Mol Cell BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment.
| ||
Nat Commun Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation. | ||
Cell Rep The Drug Vehicle and Solvent N-Methylpyrrolidone Is an Immunomodulator and Antimyeloma Compound. | ||
Elife Targeted degradation of BRD9 reverses oncogenic gene expression in synovial sarcoma. | ||
FASEB J AMPK is down-regulated by the CRL4A-CRBN axis through the polyubiquitination of AMPKα isoforms.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elisa (Verified Customer) (02-07-2022) | RPE1 cells were fixed in cold methanol for 10' at -20C . Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
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FH LUNFENG (Verified Customer) (01-27-2020) | GOOD
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FH Jing (Verified Customer) (01-27-2020) | Tested in human hiPSC-derived cardiomyocytes, it showed very clean single band around 60 kd.
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FH Yan (Verified Customer) (01-03-2020) | We tested CBRN antibody in mouse heart and brain tissue extracts. CRBN antibody have specificity to recognize CRBN by western blot which was confirmed by knockout mice.
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FH Jie (Verified Customer) (12-27-2019) | works specifically with one clear band at the right size
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FH Sarah (Verified Customer) (07-04-2019) | Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-CRBN in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.
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