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CREB1 Polyclonal antibody
CREB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA
Conjugate
Unconjugated
133
Cat no : 12208-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, rat brain tissue, multi-cells/tissue, Jurkat cells, COS-7 cells, mouse brain tissue, mouse lung tissue, HeLa cells, PC-3 cells, HL-60 cells, A431 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells, HepG2 cells, PC-12 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | mouse brain tissue, human cervical cancer tissue, human prostate cancer tissue, human thyroid tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
12208-1-AP targets CREB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Reactivity | human, mouse, rat, monkey |
Cited Reactivity | human, mouse, rat, chicken, sea cucumbers |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CREB1 fusion protein Ag2852 相同性解析による交差性が予測される生物種 |
Full Name | cAMP responsive element binding protein 1 |
Calculated molecular weight | 341 aa, 35 kDa |
Observed molecular weight | 43-46 kDa |
GenBank accession number | BC010636 |
Gene symbol | CREB1 |
Gene ID (NCBI) | 1385 |
RRID | AB_2245417 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).
Protocols
Product Specific Protocols | |
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WB protocol for CREB1 antibody 12208-1-AP | Download protocol |
IHC protocol for CREB1 antibody 12208-1-AP | Download protocol |
IF protocol for CREB1 antibody 12208-1-AP | Download protocol |
IP protocol for CREB1 antibody 12208-1-AP | Download protocol |
FC protocol for CREB1 antibody 12208-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.
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Mol Cell LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation | ||
Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. | ||
Environ Int Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms | ||
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Georgiana (Verified Customer) (01-10-2023) |
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FH Shogo (Verified Customer) (02-22-2019) | Approximately 50 mg of whole brain were applied to ChIP assay.Fragmented DNA was immunoprecipitated with the CREB antibody (250 ng) at 4 oC for overnight.
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