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CREB1 Polyclonal antibody

Name: CREB1 Polyclonal antibody
Brand: Proteintech
SKU: 12208-1-AP
Rating: 5.00 (2 reviews)

CREB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

139

Reviews (2)

Cat no : 12208-1-AP

Synonyms

Cyclic AMP-responsive element-binding protein 1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 12208-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12208-1-AP
WB result of CREB1 antibody (12208-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB1 transfected HEK-293 cells.
WB analysis using 12208-1-AP
WB result of 12208-1-AP (CREB1 antibody) with various lysates at dilution of 1:1,500.
WB analysis of rat brain using 12208-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12208-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COS-7 using 12208-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12208-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 12208-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12208-1-AP
IP result of anti-CREB1 (IP:12208-1-AP, 4ug; Detection:12208-1-AP 1:2000) with HEK-293 cells lysate 1360 ug.
IHC staining of mouse brain using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate cancer using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human thyroid using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human thyroid using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 12208-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 12208-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HEK-293 using 12208-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 12208-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human CREB1 (12208-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 12208-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, rat brain tissue, multi-cells/tissue, Jurkat cells, COS-7 cells, mouse brain tissue, mouse lung tissue, HeLa cells, PC-3 cells, HL-60 cells, A431 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells, HepG2 cells, PC-12 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse brain tissue, human cervical cancer tissue, human prostate cancer tissue, human thyroid tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HEK-293 cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 131 publications below
IHC	See 5 publications below
IF	See 4 publications below
IP	See 2 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below
ChIP	See 7 publications below
RIP	See 1 publications below

Product Information

12208-1-AP targets CREB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat, chicken, sea cucumbers
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CREB1 fusion protein Ag2852 相同性解析による交差性が予測される生物種
Full Name	cAMP responsive element binding protein 1
Calculated molecular weight	341 aa, 35 kDa
Observed molecular weight	43-46 kDa
GenBank accession number	BC010636
Gene symbol	CREB1
Gene ID (NCBI)	1385
RRID	AB_2245417
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).

Protocols

Product Specific Protocols
WB protocol for CREB1 antibody 12208-1-AP	Download protocol
IHC protocol for CREB1 antibody 12208-1-AP	Download protocol
IF protocol for CREB1 antibody 12208-1-AP	Download protocol
IP protocol for CREB1 antibody 12208-1-AP	Download protocol
FC protocol for CREB1 antibody 12208-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Cell Metab Lactate modulates iron metabolism by binding soluble adenylyl cyclase Authors - Wei Liu View Article
human	WB	Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress. Authors - Qidong Li KD Validated View Article
mouse	WB	Mol Cell LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation Authors - Shelby E Compton View Article
human	WB	Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. Authors - Bing Yao View Article
human,mouse	WB	Environ Int Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms Authors - Chaoyang Liu View Article
mouse	WB	Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke Authors - Haiyun Guo View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Georgiana (Verified Customer) (01-10-2023)	Applications: Immunofluorescence Primary Antibody Dilution: 1:500 Cell Tissue Type: Mouse brain tissue, cortex, 20um section CREB1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain tissue, cortex, 20um section (Cat no:12208-1-AP)
FH Shogo (Verified Customer) (02-22-2019)	Approximately 50 mg of whole brain were applied to ChIP assay.Fragmented DNA was immunoprecipitated with the CREB antibody (250 ng) at 4 oC for overnight. Applications: ChIP, Cell Tissue Type: Brain

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CREB1 Polyclonal antibody

CREB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 12208-1-AP

WB analysis of HEK-293 using 12208-1-AP

WB analysis using 12208-1-AP

WB analysis of rat brain using 12208-1-AP

WB analysis of Jurkat using 12208-1-AP

WB analysis of COS-7 using 12208-1-AP

WB analysis of mouse brain using 12208-1-AP

WB analysis of mouse lung using 12208-1-AP

IP experiment of HEK-293 using 12208-1-AP

IHC staining of mouse brain using 12208-1-AP

IHC staining of human cervical cancer using 12208-1-AP

IHC staining of human cervical cancer using 12208-1-AP

IHC staining of mouse brain using 12208-1-AP

IHC staining of human prostate cancer using 12208-1-AP

IHC staining of human prostate cancer using 12208-1-AP

IHC staining of human thyroid using 12208-1-AP

IHC staining of human thyroid using 12208-1-AP

IF Staining of HeLa using 12208-1-AP

IF Staining of HeLa using 12208-1-AP

IF Staining of HEK-293 using 12208-1-AP

FC experiment of HEK-293 using 12208-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Lactate modulates iron metabolism by binding soluble adenylyl cyclase

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke

Reviews

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