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  • Featured Product
  • KD/KO Validated

CREB1 Monoclonal antibody, PBS Only

CREB1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11C1

Cat no : 67927-1-PBS

Synonyms

Cyclic AMP-responsive element-binding protein 1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CREB1 Monoclonal antibody (67927-1-Ig)

    WB analysis using 67927-1-Ig (same clone as 67927-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67927-1-Ig (CREB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CREB1 Monoclonal antibody (67927-1-Ig)

    WB analysis of HEK-293 using 67927-1-Ig (same clone as 67927-1-PBS)

    WB result of CREB1 antibody (67927-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CREB1 Monoclonal antibody (67927-1-Ig)

    IHC staining of human prostate cancer using 67927-1-Ig (same clone as 67927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CREB1 Monoclonal antibody (67927-1-Ig)

    IHC staining of human cervical cancer using 67927-1-Ig (same clone as 67927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CREB1 Monoclonal antibody (67927-1-Ig)

    IHC staining of human prostate cancer using 67927-1-Ig (same clone as 67927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CREB1 Monoclonal antibody (67927-1-Ig)

    IHC staining of human cervical cancer using 67927-1-Ig (same clone as 67927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CREB1 Monoclonal antibody (67927-1-Ig)

    IF Staining of A431 using 67927-1-Ig (same clone as 67927-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CREB1 antibody (67927-1-Ig, Clone: 1E11C1 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67927-1-PBS targets CREB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CREB1 fusion protein Ag2852 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 1
Calculated molecular weight 341 aa, 35 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC010636
Gene symbol CREB1
Gene ID (NCBI) 1385
RRIDAB_2918679
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).