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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CREB3 Monoclonal antibody

CREB3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C12H11

Cat no : 68379-1-Ig

Synonyms

CREB 3, CREB3, LUMAN, LZIP

Validation Data Gallery

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  • Western Blot (WB) analysis of T-47D cells using CREB3 Monoclonal antibody (68379-1-Ig)

    WB analysis of T-47D using 68379-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HCT 116 cells using CREB3 Monoclonal antibody (68379-1-Ig)

    WB analysis of HCT 116 using 68379-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CREB3 Monoclonal antibody (68379-1-Ig)

    WB analysis using 68379-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CREB3 Monoclonal antibody (68379-1-Ig)

    IF Staining of U2OS using 68379-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CREB3 antibody (68379-1-Ig, Clone: 1C12H11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, T-47D cells, HCT 116 cells, hTERT-RPE1 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68379-1-Ig targets CREB3 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CREB3 fusion protein Ag1823 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3
Calculated molecular weight 44 kDa
Observed molecular weight 40-64 kDa
GenBank accession numberBC009402
Gene symbol CREB3
Gene ID (NCBI) 10488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

Protocols

Product Specific Protocols
WB protocol for CREB3 antibody 68379-1-IgDownload protocol
IF protocol for CREB3 antibody 68379-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols