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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CREB3L1,OASIS Monoclonal antibody (67617-1-Ig)

    WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CREB3L1,OASIS Monoclonal antibody (67617-1-Ig)

    WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CREB3L1,OASIS Monoclonal antibody (67617-1-Ig)

    WB analysis of HSC-T6 using 67617-1-Ig (same clone as 67617-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CREB3L1,OASIS Monoclonal antibody (67617-1-Ig)

    WB analysis of NIH/3T3 using 67617-1-Ig (same clone as 67617-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CREB3L1,OASIS Monoclonal antibody (67617-1-Ig)

    WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CREB3L1,OASIS Monoclonal antibody (67617-1-Ig)

    FC experiment of HepG2 using 67617-1-Ig (same clone as 67617-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50829-2

    Cytometric bead array standard curve of MP50829-2

    Cytometric bead array standard curve of MP50829-2, CREB3L1,OASIS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67617-1-PBS. Detection antibody: 67617-4-PBS. Standard:Ag28042. Range: 1.563-100 ng/mL.

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CREB3L1,OASIS Monoclonal antibody, PBS Only (Capture)

CREB3L1,OASIS Monoclonal Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 67617-1-PBS
Clone No.2B5B4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

CREB3L1, OASIS, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67617-1-PBS targets CREB3L1,OASIS as part of a matched antibody pair:

MP50829-2: 67617-1-PBS capture and 67617-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CREB3L1,OASIS fusion protein Ag28042 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3-like 1
Calculated molecular weight 57 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC015781
Gene Symbol CREB3L1/OASIS
Gene ID (NCBI) 90993
RRIDAB_2882820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96BA8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)