Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, mouse liver tissue, K-562 cells, SH-SY5Y cells, Neuro-2a cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 1 publications below |
Product Information
14514-1-AP targets CREB3L2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CREB3L2 fusion protein Ag5973 相同性解析による交差性が予測される生物種 |
| Full Name | cAMP responsive element binding protein 3-like 2 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC063666 |
| Gene Symbol | CREB3L2 |
| Gene ID (NCBI) | 64764 |
| RRID | AB_2292123 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q70SY1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclic AMP-responsive element-binding protein 3-like protein 2 (CREB3L2), also named BBF2H7, is a transcriptional activator which is activated during endoplasmic reticulum (ER) stress late phase. It has 3 isoforms produced by alternative splicing. CREB3L2 exerts protective effect in neurons by regulation of unfolded protein response (UPR)-dependent gene transcriptions. N-terminal cytoplamic domain of CREB3L2 is cleaved under ER stress and then translocates to the nucleus. Catalog#14514-1-AP is a rabbit polyclonal antibody raised against N-terminal (1-247aa) of human CREB3L2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CREB3L2 antibody 14514-1-AP | Download protocol |
| IF protocol for CREB3L2 antibody 14514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Pharmacol Azithromycin alleviates the severity of rheumatoid arthritis by targeting the unfolded protein response component of glucose-regulated protein 78 (GRP78). | ||
Int J Mol Sci Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells. | ||
J Invest Dermatol BBF2H7-mediated Sec23A pathway is required for endoplasmic reticulum-to-Golgi trafficking in dermal fibroblasts to promote collagen synthesis. | ||
J Biol Chem APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10. | ||
Mol Biol Rep Expression analysis and functional characterization of thioredoxin domain-containing protein 11 | ||