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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CREB3L2 Polyclonal antibody, PBS Only

CREB3L2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14514-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CREB3L2 Polyclonal antibody (14514-1-AP)

    WB analysis of HepG2 using 14514-1-AP (same clone as 14514-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14514-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CREB3L2 Polyclonal antibody (14514-1-AP)

    WB analysis of mouse liver using 14514-1-AP (same clone as 14514-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14514-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CREB3L2 Polyclonal antibody (14514-1-AP)

    WB analysis of mouse testis using 14514-1-AP (same clone as 14514-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14514-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CREB3L2 Polyclonal antibody (14514-1-AP)

    IF Staining of HepG2 using 14514-1-AP (same clone as 14514-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14514-1-AP (CREB3L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14514-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14514-1-PBS targets CREB3L2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREB3L2 fusion protein Ag5973 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3-like 2
Calculated molecular weight 57 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC063666
Gene symbol CREB3L2
Gene ID (NCBI) 64764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cyclic AMP-responsive element-binding protein 3-like protein 2 (CREB3L2), also named BBF2H7, is a transcriptional activator which is activated during endoplasmic reticulum (ER) stress late phase. It has 3 isoforms produced by alternative splicing. CREB3L2 exerts protective effect in neurons by regulation of unfolded protein response (UPR)-dependent gene transcriptions. N-terminal cytoplamic domain of CREB3L2 is cleaved under ER stress and then translocates to the nucleus. Catalog#14514-1-AP is a rabbit polyclonal antibody raised against N-terminal (1-247aa) of human CREB3L2.