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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CREB3L4 Polyclonal antibody (13630-1-AP)

    WB analysis using 13630-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13630-1-AP (CREB3L4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CREB3L4 Polyclonal antibody (13630-1-AP)

    WB analysis of HL-60 using 13630-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13630-1-AP (CREB3L4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CREB3L4 Polyclonal antibody (13630-1-AP)

    IF Staining of MCF-7 using 13630-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13630-1-AP (CREB3L4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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CREB3L4 Polyclonal antibody

CREB3L4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13630-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

cAMP-responsive element-binding protein 4, cAMP-responsive element-binding protein 3-like protein 4, Attaching to CRE-like 1, ATCE1, Androgen-induced basic leucine zipper protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HL-60 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13630-1-AP targets CREB3L4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREB3L4 fusion protein Ag4548 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3-like 4
Calculated molecular weight 395 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC038962
Gene Symbol CREB3L4
Gene ID (NCBI) 148327
RRIDAB_2276550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CREB3L4 (cAMP responsive element binding protein 3-like 4), also named as AIBZIP, CREB4 and ATCE1, is a member of the CREB/ATF transcription factor family. CREB3L4 may play a role in the unfolded protein response, characterized by their regulation of gene expression through the cAMP-responsive element(PMID: 22829733). CREB3L4 is highly expressed in multiple human cancers, such as human breast carcinoma, prostate cancer(PMID: 32026099, PMID: 28338058). The molecular weight of CREB3L4 is 43 kDa and 55 kDa precursor was reported. Due to glycosylation, CREB3L4 can be observed with molecular weights of 35 and 41 kDa (PMID: 25412305, 15938716).

Protocols

Product Specific Protocols
WB protocol for CREB3L4 antibody 13630-1-APDownload protocol
IF protocol for CREB3L4 antibody 13630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

iScience

CREB3L4 promotes hepatocellular carcinoma progression and decreases sorafenib chemosensitivity by promoting RHEB-mTORC1 signaling pathway

Authors - Zhengchen Jiang
  • KD Validated
View Article