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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CREBBP Polyclonal antibody (22277-1-AP)

    WB analysis using 22277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22277-1-AP (CREBBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CREBBP Polyclonal antibody (22277-1-AP)

    IHC staining of human prostate cancer using 22277-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CREBBP Polyclonal antibody (22277-1-AP)

    IHC staining of human prostate cancer using 22277-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CREBBP Polyclonal antibody (22277-1-AP)

    IF Staining of HeLa using 22277-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREBBP antibody (22277-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using CREBBP Polyclonal antibody (22277-1-AP)

    FC experiment of HeLa using 22277-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CREBBP Polyclonal antibody (22277-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CREBBP Polyclonal antibody

CREBBP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22277-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RSTS, KAT3A, CBP/p300, CBP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

22277-1-AP targets CREBBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CREB binding protein
Calculated molecular weight 265 kDa
Observed molecular weight290 kDa
GenBank accession numberNM_004380
Gene Symbol CREBBP
Gene ID (NCBI) 1387
RRIDAB_3085692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92793
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The acetyltransferase CREB-binding protein (CREBBP; also known as CBP or KAT2A) and E1A-binding protein P300 (EP300; also known as P300 or KAT2B) are two closely related regulators. Both are widely expressed within and outside the hematopoietic system and serve as tumor activators or suppressors depending on the situation. CREBBP/EP300 regulate various key physiological functions, including cell apoptosis, proliferation and differentiation, DNA repair, and somatic cell reprogramming.

Protocols

Product Specific Protocols
WB protocol for CREBBP antibody 22277-1-APDownload protocol
IHC protocol for CREBBP antibody 22277-1-APDownload protocol
IF protocol for CREBBP antibody 22277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Biosci (Landmark Ed)

Exploration of the Pharmacological Mechanism of Vitexicarpin against Triple-Negative Breast Cancer in Network Pharmacology

Authors - Tianhui Wu
View Article
humanWB

J Virol

Zinc-finger CCHC-type containing protein 8 promotes RNA virus replication by suppressing the type-I interferon responses

Authors - Shaoyu Tu
View Article