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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CRELD1 Polyclonal antibody (29903-1-AP)

    WB analysis of HepG2 using 29903-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using CRELD1 Polyclonal antibody (29903-1-AP)

    WB analysis of rat lung using 29903-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CRELD1 Polyclonal antibody (29903-1-AP)

    WB analysis of mouse lung using 29903-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CRELD1 Polyclonal antibody (29903-1-AP)

    IHC staining of mouse heart using 29903-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29903-1-AP (CRELD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CRELD1 Polyclonal antibody (29903-1-AP)

    IF Staining of U2OS using 29903-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CRELD1 antibody (29903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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CRELD1 Polyclonal antibody

CRELD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29903-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AVSD2, CIRRIN, CRELD1, DKFZp566D213

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, mouse lung tissue, rat lung tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29903-1-AP targets CRELD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRELD1 fusion protein Ag31593 相同性解析による交差性が予測される生物種
Full Name cysteine-rich with EGF-like domains 1
Calculated molecular weight45KD
Observed molecular weight45 kDa, 70-75 kDa
GenBank accession numberNM_001031717
Gene Symbol CRELD1
Gene ID (NCBI) 78987
RRIDAB_2923619
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899).

Protocols

Product Specific Protocols
WB protocol for CRELD1 antibody 29903-1-APDownload protocol
IHC protocol for CRELD1 antibody 29903-1-APDownload protocol
IF protocol for CRELD1 antibody 29903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols