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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of NIH/3T3 using 60314-1-Ig (same clone as 60314-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of RAW 264.7 using 60314-1-Ig (same clone as 60314-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of MCF-7 using 60314-1-Ig (same clone as 60314-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of COLO 320 using 60314-1-Ig (same clone as 60314-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of HSC-T6 using 60314-1-Ig (same clone as 60314-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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CREST Monoclonal antibody, PBS Only

CREST Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60314-1-PBS
Clone No.3D5D10

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, Indirect ELISA

Synonyms

CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60314-1-PBS targets CREST in WB, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CREST fusion protein Ag3119 相同性解析による交差性が予測される生物種
Full Name synovial sarcoma translocation gene on chromosome 18-like 1
Calculated molecular weight 396 aa, 43 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC034494
Gene Symbol CREST
Gene ID (NCBI) 26039
RRIDAB_2881425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75177
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).