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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26848-1-AP

Synonyms

CRH, CRF, Corticotropin-releasing hormone, Corticotropin-releasing factor, Corticotropin releasing factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRH/CRF Polyclonal antibody (26848-1-AP)

    WB analysis using 26848-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26848-1-AP (CRH/CRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IHC staining of human placenta using 26848-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IHC staining of human placenta using 26848-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IF Staining of rat brain using 26848-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CRH/CRF antibody (26848-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26848-1-AP targets CRH/CRF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRH/CRF fusion protein Ag25226 相同性解析による交差性が予測される生物種
Full Name CRH/CRF
Calculated molecular weight 21 kDa
Observed molecular weight27 kDa
GenBank accession numberBC011031
Gene symbol CRH
Gene ID (NCBI) 1392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.

Protocols

Product Specific Protocols
WB protocol for CRH/CRF antibody 26848-1-APDownload protocol
IHC protocol for CRH/CRF antibody 26848-1-APDownload protocol
IF protocol for CRH/CRF antibody 26848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols