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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated CRH/CRF Polyclonal a (CL488-10944)

    IF Staining of mouse brain using CL488-10944

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 CRH/CRF antibody (CL488-10944) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated CRH/CRF Polyclonal a (CL488-10944)

    IF Staining of mouse brain using CL488-10944

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 CRH/CRF antibody (CL488-10944) at dilution of 1:200.

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CoraLite® Plus 488-conjugated CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for IF-P
Cat No. CL488-10944

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

CRH, CRF, Corticotropin-releasing hormone, Corticotropin-releasing factor, Corticotropin releasing factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size/Concentration: 

-/ -

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Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10944 targets CRH/CRF in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRH/CRF fusion protein Ag1371 相同性解析による交差性が予測される生物種
Full Name CRH/CRF
Calculated molecular weight 21 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC011031
Gene Symbol CRH
Gene ID (NCBI) 1392
RRIDAB_3083889
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06850
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CRH/CRF antibody CL488-10944Download protocol
Standard Protocols
Click here to view our Standard Protocols