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Validation Data Gallery

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  • Western Blot (WB) analysis of rat skeletal muscle tissue using CRHR2 Polyclonal antibody (25267-1-AP)

    WB analysis of rat skeletal muscle using 25267-1-AP (same clone as 25267-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25267-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using CRHR2 Polyclonal antibody (25267-1-AP)

    WB analysis of mouse heart using 25267-1-AP (same clone as 25267-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRHR2 Polyclonal antibody (25267-1-AP)

    IHC staining of mouse brain using 25267-1-AP (same clone as 25267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRHR2 Polyclonal antibody (25267-1-AP)

    IHC staining of mouse brain using 25267-1-AP (same clone as 25267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25267-1-PBS in a different storage buffer formulation.

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CRHR2 Polyclonal antibody, PBS Only

CRHR2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25267-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Corticotropin-releasing factor receptor 2, Corticotropin-releasing hormone receptor 2, CRF R 2, CRF R2, CRF2R

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25267-1-PBS targets CRHR2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name corticotropin releasing hormone receptor 2
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberNM_001883
Gene Symbol CRHR2
Gene ID (NCBI) 1395
RRIDAB_2879996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13324
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CRHR2 is a receptor for corticotropin-releasing factor (CRF) and belongs to the G-protein coupled receptor family. It shows high affinity for CRF and also binds to related peptides such as urocortin I, II, and III. This receptor is involved in mediating the endocrine, autonomic, and behavioral responses to stress and immune challenges.