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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRIP2 Polyclonal antibody

CRIP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14801-1-AP

Synonyms

Cysteine-rich protein 2, CRP-2, CRP2, CRP 2, CRIP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using CRIP2 Polyclonal antibody (14801-1-AP)

    WB analysis of mouse heart using 14801-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CRIP2 Polyclonal antibody (14801-1-AP)

    WB analysis of HeLa using 14801-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CRIP2 Polyclonal antibody (14801-1-AP)

    WB analysis of MCF-7 using 14801-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CRIP2 Polyclonal antibody (14801-1-AP)

    WB analysis of mouse lung using 14801-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CRIP2 Polyclonal antibody (14801-1-AP)

    WB analysis of mouse brain using 14801-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CRIP2 Polyclonal antibody (14801-1-AP)

    IF Staining of HeLa using 14801-1-AP

    Immunofluorescent analysis of HeLa cells using 14801-1-AP (CRIP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CRIP2 Polyclonal antibody (14801-1-AP)

    IF Staining of HeLa using 14801-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CRIP2 antibody (14801-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, MCF-7 cells, mouse brain tissue, mouse lung tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14801-1-AP targets CRIP2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRIP2 fusion protein Ag6515 相同性解析による交差性が予測される生物種
Full Name cysteine-rich protein 2
Calculated molecular weight 22 kDa
Observed molecular weight 23-29 kDa
GenBank accession numberBC000434
Gene symbol CRIP2
Gene ID (NCBI) 1397
RRIDAB_2245316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CRIP2 antibody 14801-1-APDownload protocol
IF protocol for CRIP2 antibody 14801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF,IP

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Joleen (Verified Customer) (06-07-2019)

The CRIP2 antibody works well and the protein shows up prominently as the lower band.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
CRIP2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:14801-1-AP)