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KD/KO Validated

CRISP3 Polyclonal antibody

Name: CRISP3 Polyclonal antibody
Brand: Proteintech
SKU: 14847-1-AP

CRISP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14847-1-AP

Synonyms

Aeg2, CRISP 3, CRISP3, CRS3, SGP28

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 14847-1-AP
human saliva samples were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 14847-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14847-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14847-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 14847-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 14847-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 14847-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 14847-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of human placenta using 14847-1-AP
IP result of anti-CRISP3 (IP:14847-1-AP, 4ug; Detection:14847-1-AP 1:300) with human placenta tissue lysate 1520ug.
IHC staining of human prostate cancer using 14847-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14847-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 14847-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 14847-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 14847-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human saliva, human placenta tissue, Jurkat cells, mouse colon tissue, mouse ovary tissue, mouse thymus tissue
Positive IP detected in	human placenta tissue
Positive IHC detected in	human prostate cancer tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 3 publications below

Product Information

14847-1-AP targets CRISP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	cysteine-rich secretory protein 3
Calculated molecular weight	28 kDa
Observed molecular weight	29 kDa
GenBank accession number	NM_006061
Gene symbol	CRISP3
Gene ID (NCBI)	10321
RRID	AB_1607510
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3.

Protocols

Product Specific Protocols
WB protocol for CRISP3 antibody 14847-1-AP	Download protocol
IHC protocol for CRISP3 antibody 14847-1-AP	Download protocol
IP protocol for CRISP3 antibody 14847-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	J Natl Cancer Inst Progesterone Receptor Isoform Ratio: A Breast Cancer Prognostic and Predictive Factor for Antiprogestin Responsiveness. Authors - Paola A Rojas View Article
human	IHC	J Hematol Oncol Cysteine- rich secretory protein 3 (CRISP3), ERG and PTEN define a molecular subtype of prostate cancer with implication to patients' prognosis. Authors - Samir Al Bashir View Article
human	WB,IHC	Int J Biol Sci Circular RNA hsa_circ_0003823 promotes the Tumor Progression, Metastasis and Apatinib Resistance of Esophageal Squamous Cell Carcinoma by miR-607/CRISP3 Axis Authors - Yu-Ming Wang KD Validated View Article
human	WB	J Dent Res Proteome Analysis of Temporomandibular Joint with Disc Displacement Authors - X Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRISP3 Polyclonal antibody

CRISP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 14847-1-AP

WB analysis of human placenta using 14847-1-AP

WB analysis of Jurkat using 14847-1-AP

WB analysis of mouse thymus using 14847-1-AP

WB analysis of mouse ovary using 14847-1-AP

WB analysis of mouse ovary using 14847-1-AP

WB analysis of mouse colon using 14847-1-AP

WB analysis of mouse colon using 14847-1-AP

IP experiment of human placenta using 14847-1-AP

IHC staining of human prostate cancer using 14847-1-AP

IHC staining of human prostate cancer using 14847-1-AP

IHC staining of human pancreas using 14847-1-AP

IHC staining of human pancreas using 14847-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Progesterone Receptor Isoform Ratio: A Breast Cancer Prognostic and Predictive Factor for Antiprogestin Responsiveness.

Cysteine- rich secretory protein 3 (CRISP3), ERG and PTEN define a molecular subtype of prostate cancer with implication to patients' prognosis.

Circular RNA hsa_circ_0003823 promotes the Tumor Progression, Metastasis and Apatinib Resistance of Esophageal Squamous Cell Carcinoma by miR-607/CRISP3 Axis

Proteome Analysis of Temporomandibular Joint with Disc Displacement