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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRK Polyclonal antibody

CRK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16685-1-AP

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CRK Polyclonal antibody (16685-1-AP)

    WB analysis of NIH/3T3 using 16685-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CRK Polyclonal antibody (16685-1-AP)

    WB analysis of A549 using 16685-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CRK Polyclonal antibody (16685-1-AP)

    WB analysis of NIH/3T3 using 16685-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CRK Polyclonal antibody (16685-1-AP)

    WB analysis of PC-3 using 16685-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using CRK Polyclonal antibody (16685-1-AP)

    IHC staining of human urothelial carcinoma using 16685-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16685-1-AP (CRK antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using CRK Polyclonal antibody (16685-1-AP)

    IHC staining of human urothelial carcinoma using 16685-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16685-1-AP (CRK antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CRK Polyclonal antibody (16685-1-AP)

    IF Staining of HeLa using 16685-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16685-1-AP (CRK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CRK Polyclonal antibody (16685-1-AP)

    IF Staining of PC-3 using 16685-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 16685-1-AP (CRK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inNIH/3T3 cells, A549 cells, PC-3 cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16685-1-AP targets CRK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRK fusion protein Ag10016 相同性解析による交差性が予測される生物種
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC008506
Gene symbol CRK
Gene ID (NCBI) 1398
RRIDAB_2084893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CRK antibody 16685-1-APDownload protocol
IHC protocol for CRK antibody 16685-1-APDownload protocol
IF protocol for CRK antibody 16685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

PPIA dictates NRF2 stability to promote lung cancer progression

Authors - Weiqiang Lu
View Article