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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRK Monoclonal antibody, PBS Only

CRK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B7E1

Cat no : 67035-1-PBS

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HEK-293 using 67035-1-Ig (same clone as 67035-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of Jurkat using 67035-1-Ig (same clone as 67035-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HSC-T6 using 67035-1-Ig (same clone as 67035-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of NIH/3T3 using 67035-1-Ig (same clone as 67035-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of RAW 264.7 using 67035-1-Ig (same clone as 67035-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HeLa using 67035-1-Ig (same clone as 67035-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67035-1-PBS targets CRK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRK fusion protein Ag28793 相同性解析による交差性が予測される生物種
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC008506
Gene symbol CRK
Gene ID (NCBI) 1398
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.