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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRK Monoclonal antibody, PBS Only

CRK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B7E1

Cat no : 67035-1-PBS

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HEK-293 using 67035-1-Ig (same clone as 67035-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of Jurkat using 67035-1-Ig (same clone as 67035-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HSC-T6 using 67035-1-Ig (same clone as 67035-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of NIH/3T3 using 67035-1-Ig (same clone as 67035-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of RAW 264.7 using 67035-1-Ig (same clone as 67035-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HeLa using 67035-1-Ig (same clone as 67035-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67035-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67035-1-PBS targets CRK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRK fusion protein Ag28793 相同性解析による交差性が予測される生物種
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC008506
Gene symbol CRK
Gene ID (NCBI) 1398
RRIDAB_2882350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.