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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRLF3 Polyclonal antibody (11567-1-AP)

    WB analysis using 11567-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 11567-1-AP (CRLF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CRLF3 Polyclonal antibody

CRLF3 Polyclonal Antibody for WB, ELISA
Cat No. 11567-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CREME 9, CREME9, CRLF3, CYTOR4, FRWS, P48

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11567-1-AP targets CRLF3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRLF3 fusion protein Ag2144 相同性解析による交差性が予測される生物種
Full Name cytokine receptor-like factor 3
Calculated molecular weight 442 aa, 50 kDa
Observed molecular weight51 kDa
GenBank accession numberBC023567
Gene Symbol CRLF3
Gene ID (NCBI) 51379
RRIDAB_3085377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUI8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytokine receptor-like factor 3 (CRLF3), a member of the group 1 in the prototypic class I cytokine receptors, contains the conserved cytokine receptor motif WSXWS, a single transmembrane segment and an intracellular Janus kinase (JAK) docking site (PMID: 31680856). CRLF3 is expressed in most normal human tissues and many tumor cell lines and has been reported to negatively regulate cell cycle progression (PMID: 19427400). CRLF3 has been identified as a human neuroprotective EV-3 (Epo) receptor (PMID: 37168680). It is involved neurogenesis, neuron survival, and dendritic development (PMID: 34233200).

Protocols

Product Specific Protocols
WB protocol for CRLF3 antibody 11567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols