CRM1 Polyclonal antibody
CRM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 27917-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, C6 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, K-562 cells, HT-1080 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
27917-1-AP targets CRM1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CRM1 fusion protein Ag27207 相同性解析による交差性が予測される生物種 |
| Full Name | exportin 1 (CRM1 homolog, yeast) |
| Calculated molecular weight | 1071 aa, 123 kDa |
| Observed molecular weight | 123 kDa |
| GenBank accession number | BC032847 |
| Gene symbol | CRM1 |
| Gene ID (NCBI) | 7514 |
| RRID | AB_2881009 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRM1 antibody 27917-1-AP | Download protocol |
| IHC protocol for CRM1 antibody 27917-1-AP | Download protocol |
| IF protocol for CRM1 antibody 27917-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol YTHDC1/CRM1 Facilitates m6A-Modified circRNA388 Nuclear Export to Induce Coelomocyte Autophagy via the miR-2008/ULK Axis in Apostichopus japonicus | ||
J Cell Commun Signal CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles | ||
J Cancer Res Clin Oncol Selinexor demonstrates anti-tumor efficacy in paired patient-derived xenograft models and hydrogel-embedded histoculture drug sensitivity test of penile cancer | ||
Mol Cell EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma | ||
Mol Cell A GAPDH serotonylation system couples CD8+ T cell glycolytic metabolism to antitumor immunity |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xingyuan (Verified Customer) (07-17-2023) | The band quality was pretty good
 |
FH Wei (Verified Customer) (03-12-2020) | strong, clear band for mouse heart lysates
|
