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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRM1 Monoclonal antibody, PBS Only

CRM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D3B6

Cat no : 66763-1-PBS

Synonyms

CRM1, DKFZp686B1823, Exp1, Exportin 1, XPO1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRM1 Monoclonal antibody (66763-1-Ig)

    WB analysis using 66763-1-Ig (same clone as 66763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CRM1 Monoclonal antibody (66763-1-Ig)

    WB analysis using 66763-1-Ig (same clone as 66763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CRM1 Monoclonal antibody (66763-1-Ig)

    IHC staining of human breast cancer using 66763-1-Ig (same clone as 66763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CRM1 Monoclonal antibody (66763-1-Ig)

    IHC staining of human breast cancer using 66763-1-Ig (same clone as 66763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CRM1 Monoclonal antibody (66763-1-Ig)

    IF Staining of HeLa using 66763-1-Ig (same clone as 66763-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CRM1 antibody (66763-1-Ig, Clone: 2D3B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66763-1-PBS targets CRM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRM1 fusion protein Ag27337 相同性解析による交差性が予測される生物種
Full Name exportin 1 (CRM1 homolog, yeast)
Calculated molecular weight 1071 aa, 123 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC032847
Gene symbol CRM1
Gene ID (NCBI) 7514
RRIDAB_2882109
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).