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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRMP1 Polyclonal antibody (29497-1-AP)

    WB analysis using 29497-1-AP (same clone as 29497-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 29497-1-AP (CRMP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP1 Polyclonal antibody (29497-1-AP)

    IHC staining of mouse brain using 29497-1-AP (same clone as 29497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29497-1-AP (CRMP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP1 Polyclonal antibody (29497-1-AP)

    IHC staining of mouse brain using 29497-1-AP (same clone as 29497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29497-1-AP (CRMP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29497-1-PBS in a different storage buffer formulation.

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CRMP1 Polyclonal antibody, PBS Only

CRMP1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29497-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29497-1-PBS targets CRMP1 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRMP1 fusion protein Ag31559 相同性解析による交差性が予測される生物種
Full Name collapsin response mediator protein 1
Calculated molecular weight62KD
Observed molecular weight62 kDa
GenBank accession numberNM_001014809
Gene Symbol CRMP1
Gene ID (NCBI) 1400
RRIDAB_2918317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14194
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62-kDa protein and its 75-kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.