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KD/KO Validated

CROP Polyclonal antibody

Name: CROP Polyclonal antibody
Brand: Proteintech
SKU: 14504-1-AP

CROP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14504-1-AP

Synonyms

CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of Jurkat using 14504-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14504-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14504-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14504-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14504-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14504-1-AP
IP result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug.
IHC staining of human ovary cancer using 14504-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14504-1-AP (CROP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary using 14504-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 14504-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human pancreas cancer using 14504-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas cancer using 14504-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 14504-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (14504-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Cat No. 14504-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HepG2 cells, mouse brain tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human ovary cancer tissue, human ovary tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 1 publications below
IP	See 1 publications below

Product Information

14504-1-AP targets CROP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CROP fusion protein Ag5961 相同性解析による交差性が予測される生物種
Full Name	cisplatin resistance-associated overexpressed protein
Calculated molecular weight	51 kDa
Observed molecular weight	55-58 kDa
GenBank accession number	BC056409
Gene symbol	CROP
Gene ID (NCBI)	51747
RRID	AB_2229967
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

Protocols

Product Specific Protocols
WB protocol for CROP antibody 14504-1-AP	Download protocol
IHC protocol for CROP antibody 14504-1-AP	Download protocol
IF protocol for CROP antibody 14504-1-AP	Download protocol
IP protocol for CROP antibody 14504-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	PLoS Genet Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors. Authors - Emily M Schleicher KD Validated View Article
human	WB	Cell Rep Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms. Authors - Noah J Daniels KD Validated View Article
human	WB,IP	Cell Insight LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability Authors - Xiaqing Zhang View Article
human	WB	Curr Issues Mol Biol Proteomics Identifies LUC7L3 as a Prognostic Biomarker for Hepatocellular Carcinoma Authors - Yushan Hou View Article
mouse	IHC	Inorg Chem Studies of Anticancer Activities In Vitro and In Vivo for Butyltin(IV)-Iridium(III) Imidazole-Phenanthroline Complexes with Aggregation-Induced Emission Properties Authors - Yiwei Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CROP Polyclonal antibody

CROP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 14504-1-AP

WB analysis of HepG2 using 14504-1-AP

WB analysis of mouse brain using 14504-1-AP

WB analysis of HepG2 using 14504-1-AP

WB analysis of Jurkat using 14504-1-AP

IP experiment of HepG2 using 14504-1-AP

IHC staining of human ovary cancer using 14504-1-AP

IHC staining of human ovary using 14504-1-AP

IHC staining of human ovary using 14504-1-AP

IHC staining of human pancreas cancer using 14504-1-AP

IHC staining of human pancreas cancer using 14504-1-AP

IF Staining of HepG2 using 14504-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms.

LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability

Proteomics Identifies LUC7L3 as a Prognostic Biomarker for Hepatocellular Carcinoma

Studies of Anticancer Activities In Vitro and In Vivo for Butyltin(IV)-Iridium(III) Imidazole-Phenanthroline Complexes with Aggregation-Induced Emission Properties