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  • KD/KO Validated

CROT Polyclonal antibody

CROT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13543-1-AP

Synonyms

Peroxisomal carnitine O-octanoyltransferase, EC:2.3.1.137, COT


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Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13543-1-AP targets CROT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CROT fusion protein Ag4486 相同性解析による交差性が予測される生物種
Full Name carnitine O-octanoyltransferase
Calculated molecular weight 612 aa, 66 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC039004
Gene symbol CROT
Gene ID (NCBI) 54677
RRIDAB_2085513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CROT, also named as COT, belongs to the carnitine/choline acetyltransferase family. It is a beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. CROT converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CROT is an active forms for carnitine octanoyltransferase. This antibody can bind the close sequences genes.

Protocols

Product Specific Protocols
WB protocol for CROT antibody 13543-1-APDownload protocol
IHC protocol for CROT antibody 13543-1-APDownload protocol
IF protocol for CROT antibody 13543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Front Endocrinol (Lausanne)

The miR-33a-5p/CROT axis mediates ovarian cancer cell behaviors and chemoresistance via the regulation of the TGF-β signal pathway

Authors - Xin Li
  • KD Validated
humanWB

J Biol Chem

Carnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cells

Authors - Jake Hsu
  • KD Validated
humanWB

J Biol Chem

Carnitine o-octanoyltransferase is a p53 target that promotes oxidative metabolism and cell survival following nutrient starvation

Authors - Jack D Sanford
  • KD Validated
mouseWB

Am J Transl Res

Artemether regulates liver glycogen and lipid utilization through mitochondrial pyruvate oxidation in db/db mice

Authors - Wenci Weng
mouseWB

iScience

Etomoxir repurposed as a promiscuous fatty acid mimetic chemoproteomic probe

Authors - Joseph Choi