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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRP Monoclonal antibody, PBS Only

CRP Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F10A11

Cat no : 66250-1-PBS

Synonyms

C reactive protein, C-Reactive Protein, CRP, PTX1

Validation Data Gallery

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  • Western Blot (WB) analysis of serum from mouse injected with bacteria using CRP Monoclonal antibody (66250-1-Ig)

    WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP Antibody) at dilution of 1:2000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of serum from mouse injected with bacteria using CRP Monoclonal antibody (66250-1-Ig)

    WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    Serum from normal mouse (left) and mouse injected with bacteria (right) were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP Antibody) at dilution of 1:1000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using CRP Monoclonal antibody (66250-1-Ig)

    WB analysis of pig liver using 66250-1-Ig (same clone as 66250-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using CRP Monoclonal antibody (66250-1-Ig)

    WB analysis of rat liver using 66250-1-Ig (same clone as 66250-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CRP Monoclonal antibody (66250-1-Ig)

    IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (CRP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CRP Monoclonal antibody (66250-1-Ig)

    IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (CRP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CRP Monoclonal antibody (66250-1-Ig)

    IF Staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66250-1-Ig (CRP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66250-1-PBS targets CRP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CRP fusion protein Ag9883 相同性解析による交差性が予測される生物種
Full Name C-reactive protein, pentraxin-related
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC020766
Gene symbol CRP
Gene ID (NCBI) 1401
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.