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Validation Data Gallery

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  • Western Blot (WB) analysis of serum from mouse injected with bacteria using C-Reactive Protein/CRP Monoclonal antibody (66250-1-Ig)

    WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:2000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of serum from mouse injected with bacteria using C-Reactive Protein/CRP Monoclonal antibody (66250-1-Ig)

    WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    Serum from normal mouse (left) and mouse injected with bacteria (right) were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:1000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using C-Reactive Protein/CRP Monoclonal antibody (66250-1-Ig)

    WB analysis of pig liver using 66250-1-Ig (same clone as 66250-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using C-Reactive Protein/CRP Monoclonal antibody (66250-1-Ig)

    WB analysis of rat liver using 66250-1-Ig (same clone as 66250-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using C-Reactive Protein/CRP Monoclonal antibody (66250-1-Ig)

    IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using C-Reactive Protein/CRP Monoclonal antibody (66250-1-Ig)

    IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using C-Reactive Protein/CRP Monoclonal antibody (66250-1-Ig)

    IF Staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66250-1-Ig (CRP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

C-Reactive Protein/CRP Monoclonal antibody, PBS Only

C-Reactive Protein/CRP Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66250-1-PBS
Clone No.3F10A11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66250-1-PBS targets C-Reactive Protein/CRP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen C-Reactive Protein/CRP fusion protein Ag9883 相同性解析による交差性が予測される生物種
Full Name C-reactive protein, pentraxin-related
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC020766
Gene Symbol CRP
Gene ID (NCBI) 1401
RRIDAB_2881638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02741
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.