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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRTAC1 Polyclonal antibody

CRTAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30251-1-AP

Synonyms

ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRTAC1 Polyclonal antibody (30251-1-AP)

    WB analysis using 30251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CRTAC1 Polyclonal antibody (30251-1-AP)

    WB analysis using 30251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRTAC1 Polyclonal antibody (30251-1-AP)

    IHC staining of mouse brain using 30251-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CRTAC1 Polyclonal antibody (30251-1-AP)

    IHC staining of rat brain using 30251-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman plasma, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30251-1-AP targets CRTAC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRTAC1 fusion protein Ag32963 相同性解析による交差性が予測される生物種
Full Name cartilage acidic protein 1
Calculated molecular weight 661 aa, 71 kDa
Observed molecular weight 71-100 kDa
GenBank accession numberBC034245
Gene symbol CRTAC1
Gene ID (NCBI) 55118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRTAC1 (Cartilage acidic protein 1) is also named as ASPIC and 68 kDa chondrocyte-expressed protein (CEP-68). Cartilage acidic protein 1 (CRTAC1), a novel human marker which allowed discrimination of human chondrocytes from osteoblasts and mesenchymal stem cells (PMID: 17074475). CRTAC1 is a glycosylated calcium-binding extracellular matrix protein (PMID: 37796703). CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage (PMID: 17074475). CRTAC1 was downregulated in bladder cancer tissues and cells (PMID: 34818994).

Protocols

Product Specific Protocols
WB protocol for CRTAC1 antibody 30251-1-APDownload protocol
IHC protocol for CRTAC1 antibody 30251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols