Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, rabbit cerebellum tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68276-1-Ig targets CRTAC1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse, rabbit, pig samples.
| Tested Reactivity | Human, rat, mouse, rabbit, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CRTAC1 fusion protein Ag32813 相同性解析による交差性が予測される生物種 |
| Full Name | cartilage acidic protein 1 |
| Calculated molecular weight | 661 aa, 71 kDa |
| Observed molecular weight | 71 kDa |
| GenBank accession number | BC034245 |
| Gene Symbol | CRTAC1 |
| Gene ID (NCBI) | 55118 |
| RRID | AB_2935358 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NQ79 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRTAC1 (cartilage acidic protein 1) is also named as ASPIC1 and CEP68. CRTAC1 is an extracellular matrix protein of human chondrogenic tissue. CRTAC1 promotes cell proliferation, migration and extracellular matrix regeneration and remodeling in primary human fibroblasts (PMID:32084494). CRTAC1 expression is decreased in tissue samples of bladder urothelial carcinoma (PMID:34818994). CRTAC1 overexpression promotes the pyroptosis of human lens epithelial cells by facilitating the reactive oxygen species (ROS) production in the formation of cataract (PMID:32838966). CRTAC1 was the most compelling and robust biomarker for osteoarthritis severity and progression (PMID:35924962).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRTAC1 antibody 68276-1-Ig | Download protocol |
| IF protocol for CRTAC1 antibody 68276-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |