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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRTAM Polyclonal antibody

CRTAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16627-1-AP

Synonyms

Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRTAM Polyclonal antibody (16627-1-AP)

    WB analysis using 16627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16627-1-AP (CRTAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, mouse thymus tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16627-1-AP targets CRTAM in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRTAM fusion protein Ag9956 相同性解析による交差性が予測される生物種
Full Name cytotoxic and regulatory T cell molecule
Calculated molecular weight 393 aa, 45 kDa
Observed molecular weight70 kDa
GenBank accession numberBC070266
Gene symbol CRTAM
Gene ID (NCBI) 56253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223). The predicted molecular weight of CRTAM is 43 kDa. Due to glycosylation, the observed molecular weight of CRTAM can be apparent at ~80 kDa (PMID: 16300832).

Protocols

Product Specific Protocols
WB protocol for CRTAM antibody 16627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols