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KD/KO Validated

CRTC2,TORC2 Polyclonal antibody

Name: CRTC2,TORC2 Polyclonal antibody
Brand: Proteintech
SKU: 12497-1-AP

CRTC2,TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12497-1-AP

Synonyms

CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC2

Validation Data Gallery

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AllWBIHCIF/ICC

WB analysis using 12497-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12497-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12497-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12497-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 12497-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of rat kidney using 12497-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 12497-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 12497-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 12497-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12497-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12497-1-AP
Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HeLa using 12497-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 12497-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse liver tissue, HEK-293 cells, mouse kidney, rat kidney
Positive IHC detected in	human skin cancer tissue, human gliomas tissue, rat kidney tissue, human urothelial carcinoma tissue, mouse lung tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 17 publications below
IHC	See 5 publications below
IF	See 7 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

12497-1-AP targets CRTC2,TORC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CRTC2,TORC2 fusion protein Ag3167 相同性解析による交差性が予測される生物種
Full Name	CREB regulated transcription coactivator 2
Calculated molecular weight	693 aa, 73 kDa
Observed molecular weight	73 kDa
GenBank accession number	BC053562
Gene symbol	TORC2
Gene ID (NCBI)	200186
RRID	AB_2260910
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

Protocols

Product Specific Protocols
WB protocol for CRTC2,TORC2 antibody 12497-1-AP	Download protocol
IHC protocol for CRTC2,TORC2 antibody 12497-1-AP	Download protocol
IF protocol for CRTC2,TORC2 antibody 12497-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,IP	Adv Sci (Weinh) cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease. Authors - Zeyun Mi KD Validated View Article
mouse	WB	Mol Cell LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation Authors - Shelby E Compton View Article
human	IHC	Am J Transplant The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside. Authors - Qi Ling View Article
mouse	IHC,IF	J Neurosci Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock. Authors - Sakamoto Kensuke K View Article
human,mouse,bovine	WB,IF	J Neurosci CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function. Authors - Hideaki Kanki View Article
human	WB,IF	J Virol Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells. Authors - Yuan Jinxiang J View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRTC2,TORC2 Polyclonal antibody

CRTC2,TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 12497-1-AP

WB analysis of mouse liver using 12497-1-AP

WB analysis of mouse liver using 12497-1-AP

WB analysis of HEK-293 using 12497-1-AP

IHC staining of human gliomas using 12497-1-AP

IHC staining of rat kidney using 12497-1-AP

IHC staining of human skin cancer using 12497-1-AP

IHC staining of human urothelial carcinoma using 12497-1-AP

IHC staining of mouse lung using 12497-1-AP

IHC staining of mouse kidney using 12497-1-AP

IF Staining of HeLa using 12497-1-AP

IF Staining of HeLa using 12497-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.

LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation

The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.

Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock.

CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function.

Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells.

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