• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRTC3 Polyclonal antibody

CRTC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25476-1-AP

Synonyms

Transducer of CREB protein 3, TORC3, TORC 3, CRTC 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using CRTC3 Polyclonal antibody (25476-1-AP)

    WB analysis of mouse brain using 25476-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25476-1-AP (CRTC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CRTC3 Polyclonal antibody (25476-1-AP)

    WB analysis of MCF-7 using 25476-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25476-1-AP (CRTC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CRTC3 Polyclonal antibody (25476-1-AP)

    WB analysis of mouse brain using 25476-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25476-1-AP (CRTC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CRTC3 Polyclonal antibody (25476-1-AP)

    IHC staining of human stomach cancer using 25476-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25476-1-AP (CRTC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CRTC3 Polyclonal antibody (25476-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25476-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25476-1-AP (CRTC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25476-1-AP targets CRTC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CREB regulated transcription coactivator 3
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberNM_022769
Gene symbol CRTC3
Gene ID (NCBI) 64784
RRIDAB_2880097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CRTC3 antibody 25476-1-APDownload protocol
IHC protocol for CRTC3 antibody 25476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols