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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRX Monoclonal antibody, PBS Only

CRX Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3C11

Cat no : 67555-1-PBS

Synonyms

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, LCA7, OTX3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRX Monoclonal antibody (67555-1-Ig)

    WB analysis using 67555-1-Ig (same clone as 67555-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67555-1-Ig (CRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67555-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using CRX Monoclonal antibody (67555-1-Ig)

    WB analysis of Y79 using 67555-1-Ig (same clone as 67555-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 67555-1-Ig (CRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67555-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CRX Monoclonal antibody (67555-1-Ig)

    IHC staining of mouse eye using 67555-1-Ig (same clone as 67555-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67555-1-Ig (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67555-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67555-1-PBS targets CRX in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRX fusion protein Ag30086 相同性解析による交差性が予測される生物種
Full Name cone-rod homeobox
Calculated molecular weight 299 aa, 32 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC016664
Gene symbol CRX
Gene ID (NCBI) 1406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].