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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cryptochrome 1 Recombinant antibody, PBS Only

Cryptochrome 1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240520B12

Cat no : 83602-5-PBS

Synonyms

CRY1, Cryptochrome-1, CRY 1, 240520B12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cryptochrome 1 Recombinant antibody (83602-5-RR)

    WB analysis using 83602-5-RR (same clone as 83602-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83602-5-RR (CRY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83602-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83602-5-RR

    Affinity and Kinetic Characterization of 83602-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83602-5-RR against Human Cryptochrome 1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83602-5-PBS is Cryptochrome 1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cryptochrome 1 fusion protein Ag4270 相同性解析による交差性が予測される生物種
Full Name cryptochrome 1 (photolyase-like)
Calculated molecular weight 586 aa, 66 kDa
Observed molecular weight60-66 kDa
GenBank accession numberBC030519
Gene symbol CRY1
Gene ID (NCBI) 1407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481).