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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRYBB3 Polyclonal antibody

CRYBB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21009-1-AP

Synonyms

Beta B3 crystallin, Beta crystallin B3, CATCN2, CRYB3, CRYBB3, crystallin, beta B3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRYBB3 Polyclonal antibody (21009-1-AP)

    WB analysis using 21009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21009-1-AP (CRYBB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CRYBB3 Polyclonal antibody (21009-1-AP)

    IHC staining of mouse eye using 21009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21009-1-AP (CRYBB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CRYBB3 Polyclonal antibody (21009-1-AP)

    IHC staining of mouse eye using 21009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21009-1-AP (CRYBB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse eye tissue, rat eye tisssue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 21009-1-AP is CRYBB3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRYBB3 fusion protein Ag15212 相同性解析による交差性が予測される生物種
Full Name crystallin, beta B3
Calculated molecular weight 211 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC069479
Gene symbol CRYBB3
Gene ID (NCBI) 1417
RRIDAB_10732686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CRYBB3 antibody 21009-1-APDownload protocol
IHC protocol for CRYBB3 antibody 21009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Lab Invest

Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway.

Authors - Wenting Yu
View Article