Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16131-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16131-1-AP (Citrate synthase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16131-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16131-1-AP (Citrate synthase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16131-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16131-1-AP (Citrate synthase antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16131-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16131-1-AP (Citrate synthase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 16131-1-AP
IP result of anti-Citrate synthase (IP:16131-1-AP, 4ug; Detection:16131-1-AP 1:1000) with mouse heart tissue lysate 3200ug.
IHC staining of human liver cancer using 16131-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16131-1-AP (Citrate synthase antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16131-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16131-1-AP (Citrate synthase antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16131-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Citrate synthase antibody (16131-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
IF Staining of HepG2 using 16131-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16131-1-AP (Citrate synthase antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Citrate synthase Polyclonal antibody

Citrate synthase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16131-1-AP

Publications(88)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CS, CISY, Citrate, Citrate synthase, mitochondrial, EC:2.3.3.1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (88)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse heart tissue, HepG2 cells, MDA-MB-231 cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 73 publications below
IHC	See 4 publications below
IF	See 10 publications below
IP	See 3 publications below
CoIP	See 1 publications below

Product Information

16131-1-AP targets Citrate synthase in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit, chicken, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9117 Product name: Recombinant human CS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-466 aa of BC010106 Sequence: EQVSWLSKEWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYAQGISRTKYWELIYEDSMDLIAKLPCVAAKIYRNLYREGSGIGAIDSNLDWSHNFTNMLGYTDHQFTELTRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPMFKLVAQLYKIVPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTEGLMKFVDSKSG 相同性解析による交差性が予測される生物種
Full Name	citrate synthase
Calculated molecular weight	466 aa, 52 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC010106
Gene Symbol	Citrate Synthase
Gene ID (NCBI)	1431
RRID	AB_1640013
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75390
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

Protocols

Product Specific Protocols
IF protocol for Citrate synthase antibody 16131-1-AP	Download protocol
IHC protocol for Citrate synthase antibody 16131-1-AP	Download protocol
IP protocol for Citrate synthase antibody 16131-1-AP	Download protocol
WB protocol for Citrate synthase antibody 16131-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration Authors - Zhuo Li View Article
human	WB	Nat Cell Biol Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis. Authors - Miranda Y Fong View Article
mouse	IF	Nat Neurosci A glycolytic shift in Schwann cells supports injured axons. Authors - Elisabetta Babetto View Article
human	IF	ACS Cent Sci A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen Species Authors - Cong Tang View Article
human	IF	Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. Authors - Bin Jiang View Article
human	WB	Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Authors - Xiaoyu Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16131-1-AP

WB analysis of mouse heart using 16131-1-AP

WB analysis of HeLa using 16131-1-AP

WB analysis of HeLa using 16131-1-AP

IP experiment of mouse heart using 16131-1-AP

IHC staining of human liver cancer using 16131-1-AP

IHC staining of human liver cancer using 16131-1-AP

IF Staining of HepG2 using 16131-1-AP

IF Staining of HepG2 using 16131-1-AP

Citrate synthase Polyclonal antibody

Citrate synthase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.

A glycolytic shift in Schwann cells supports injured axons.

A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen Species

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.