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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSDA Polyclonal antibody (31704-1-AP)

    WB analysis using 31704-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 31704-1-AP (CSDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of KYSE-30 cells using CSDA Polyclonal antibody (31704-1-AP)

    WB analysis of KYSE-30 using 31704-1-AP

    WB result of CSDA antibody (31704-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CSDA knockout KYSE-30 cells.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CSDA Polyclonal antibody (31704-1-AP)

    IF Staining of U2OS using 31704-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CSDA antibody (31704-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CSDA Polyclonal antibody

CSDA Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31704-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Y-box-binding protein 3, Single-strand DNA-binding protein NF-GMB, DNA-binding protein A, DBPA, Cold shock domain-containing protein A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, KYSE-30 cells, mouse liver tissue, mouse skeletal muscle tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31704-1-AP targets CSDA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSDA fusion protein Ag36519 相同性解析による交差性が予測される生物種
Full Name cold shock domain protein A
Calculated molecular weight 40 kDa
Observed molecular weight60-68 kDa
GenBank accession numberBC008801
Gene Symbol CSDA
Gene ID (NCBI) 8531
RRIDAB_3670088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP16989
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791).

Protocols

Product Specific Protocols
WB protocol for CSDA antibody 31704-1-APDownload protocol
IF protocol for CSDA antibody 31704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols