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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSDC2 Recombinant antibody

CSDC2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241031A11

Cat no : 83886-6-RR

Synonyms

RNA-binding protein PIPPin, RNA binding protein PIPPin, PIPPIN, Cold shock domain-containing protein C2, 241031A11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CSDC2 Recombinant antibody (83886-6-RR)

    IF Staining of HeLa using 83886-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSDC2 antibody (83886-6-RR, Clone: 241031A11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U-2 OS using CSDC2 Recombinant antibody (83886-6-RR)

    FC experiment of U-2 OS using 83886-6-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug CSDC2 Recombinant antibody (83886-6-RR, Clone:241031A11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83886-6-RR

    Affinity and Kinetic Characterization of 83886-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83886-6-RR against Human CSDC2 were performed. The affinity constant is 0.256 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-2 OS

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83886-6-RR is CSDC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CSDC2 fusion protein Ag16216 相同性解析による交差性が予測される生物種
Full Name cold shock domain containing C2, RNA binding
Calculated molecular weight 153 aa, 17 kDa
GenBank accession numberBC067113
Gene symbol CSDC2
Gene ID (NCBI) 27254
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIPPIN, also known as CSDC2 (Cold shock domain-containing protein C2) is an RNA‐binding protein (RBP), highly enriched in the rat brain, specifically enriched in some pyramidal neurons of the cerebral cortex and in the Purkinje cells of the cerebellum (PMID: 10446180). PIPPIN has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Nuclear CSDC2 is connected to proliferation and cytoplasmic CSDC2 to terminal differentiation in the decidua and that CSDC2 could regulate differentiation during decidua development (PMID: 30078185, PMID: 17053029).

Protocols

Product Specific Protocols
IF protocol for CSDC2 antibody 83886-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols