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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSF1R Recombinant antibody

CSF1R Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241675D4

Cat no : 84477-6-RR

Synonyms

CSF-1R, CSF-1 receptor, CSF 1 R, CD115, C FMS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSF1R Recombinant antibody (84477-6-RR)

    WB analysis using 84477-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84477-6-RR (CSF1R antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CSF1R Recombinant antibody (84477-6-RR)

    IF Staining of THP-1 using 84477-6-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF1R antibody (84477-6-RR, Clone: 241675D4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inTHP-1 cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84477-6-RR targets CSF1R in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 1 receptor
Calculated molecular weight 972 aa, 108 kDa
Observed molecular weight160 kDa
GenBank accession numberBC047521
Gene symbol CSF1R
Gene ID (NCBI) 1436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

Protocols

Product Specific Protocols
WB protocol for CSF1R antibody 84477-6-RRDownload protocol
IF protocol for CSF1R antibody 84477-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols